Explore upstream bioprocessing solutions in the biopharma industry, focusing on cell culture techniques, fermentation methods, and media optimization strategies for enhanced productivity.
Navigate the complexity of upstream bioprocessing solutions, revealing fundamental principles and advancement strategies that rise above state-of-the-art capabilities in the biopharma industry.
Cell Culture Techniques:
Cell Nature & Product Era: Cultured Suspension adherent SciFlow Cell suspensions where cells are cultured freely in culture medium are frequently chosen because of scalability, while adhesion among cells and the cell adhesion surface is necessary for the use of some cell types.
Bioreactor Systems: Bioreactors are important components for cell culture as they give a controlled environment for the growth of cells. Various options include Stirred-tank bioreactors, Wave bioreactors, perfusion systems etc. Which system is right for you will depend on things like cell-type, scalability and process requirements.
Fermentation Techniques:
Modes of Fermentation: The modes can be categorized as Batch, fed-batch and continuous fermentation. These processes differ only in the fact that batch fermentation does not allow for the addition of nutrients during the process, and fed-batch does. Continuous fermentation delivers a stable-state, which can have benefits in both productivity and utilities consumption.
Oxygenation/Aeration and Agitation: Oxygen is essential for microbial growth and to achieve the best product yield, adequate aeration (4–15% O2) combined with controlled agitation within the fermentor are necessary. Oxygen availability, mixing efficiency, and shear stress on cells all play important roles in industrial fermentation system design. All this leads to a well balanced condition for best performance of the microbes.
Media Optimization:
Media Optimization: Nutrient Composition studies/media optimization studies are carried in this field of research. We must optimize nutrient composition for meeting nutrient requirements of individual cell or microorganism. These are carbon sources, nitrogen sources, minerals and vitamins. A better and a more effective media leads to improved cell growth and higher productivity.
Feed Strategies: The application of feed strategies such as intermittent or continuous feeding are crucial for controlling nutrient levels during the bioprocess. This guarantees that the growth of the cell and subsequently also product assembly is consistent.
Conclusion: Advancing Biomanufacturing
The production of biotherapeutics is transparent by a number of steps which are collectively termed “upstream bioprocessing”. The biopharmaceutical industry will improve product quality, production yields, and processes as a whole by mastering the principles of cell culture, fermentation, and media optimization.
BioBoston Consulting, pioneers in the space are prepared to partner with key industry stakeholders providing customized solutions and capability to advance upstream bioprocessing. Join us in our endeavor to advance the frontiers of innovation and excellence in biomanufacturing.
Contact BioBoston Consulting or visit our website to find out how we can help your organization.
